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10.1073/pnas.0800019105 5 BayrH.DixonS

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were resuspended in lysis buffer [20 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM imidazole, 1 mM DTT)] containing 0.5 mg/ml lysozyme

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GB, also called as original betaine (N,N,N-trimethylglycine) was first discovered from Beta vulgaris which is later found to be distributed in microorganisms, plants and animals